Towbin, without SDS, 10X Western blot transfer buffer for use with nitrocellulose and PVDF transfer membranes, pH 8.3. Recommended for use when transferring proteins that are highly soluble and have proven to elute from gels easily.

Towbin, without SDS, 10X

Towbin, without SDS, 10X is rated 5.0 out of 5 by 1.
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Application: A western blot transfer buffer for nitrocellulose and PVDF membranes
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
Towbin, without SDS, 10X sc-24955 1 L $30.00

Towbin, without SDS is a western blot transfer buffer for use with nitrocellulose and PVDF transfer membranes. Towbin buffer is a Tris-glycine buffer with a pH of 8.3.This product is recommended for use when transferring proteins that are highly soluble and have proven to elute from gels easily. If protein transfer is inefficient, a suitable alternative may be: Towbin, with SDS, 10X (sc-24954).


References

1. Towbin, H., et al. 1979. Proc. Natl. Acad. Sci. U.S.A. 76: 4350-4354. PMID: 388439
2. Towbin, H., et al. 1992. Biotechnology. 24: 145-149. PMID: 1422008

Usage :
• Dilute to a 1X solution with ddH20.
• If desired, methanol may be added to the transfer buffer (suggest 10 - 20% concentration).
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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Rated 5 out of 5 by from Various publications cite Towbin (without Various publications cite Towbin (without SDS) as a Western blot transfer buffer for use with nitrocellulose and PVDF transfer membranes. Recommended for use when transferring proteins that are highly soluble and have proven to elute from gels easily. -SCBT Publication Review
Date published: 2015-03-22
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